Biology II – Biotechnology Chapters 14.3, 15

Extra Credit: Biology Junction Crossword

 

14.3 Studying the Human Genome I

Human Genome Project Animation: http://www.yourgenome.org/landing_teachers.shtml (HGP info sheet & DNA to Data worksheet)

14.3 PowerPoint – 33 slides (restriction enzyme, gel electrophoresis, bioinformatics, genomics)

 

Summarize the methods of DNA analysis

State the Goals of the Human Genome Project and explain what we have learned so far

Quick Lab: Modeling Restriction Enzymes – textbook page 405

Homework: 14.3 Study Workbook (and chapter 14 vocab review for review)

 

**Biology Junction DNA Technology PPT slides 1-11(DNA Extraction, Steps in DNA Sequencing), slides 20-23 (Human Genome Project)

NOVA: Cracking the Code of Life http://www.pbs.org/wgbh/nova/genome/

2hour video: 1. instructions for a human being 2. getting the code out 3. one wrong letter 4. the sequencing race begins 5. ramping up 6. genetic variation 7. who owns the genome 8. the business of science 9. finding the cure is hard 10. complexity in proteins 11. the finish line 12. finding disease gene 13 DNA database 14. a family disease 15. genetic modification 16. contemplating the message 

go over 14.3 Homework

 

14.3 Studying the Human Genome II

BioGlix: Molecular Genetics & the Human Genome(30 minutes)

DNA Sequencing Activity

Learn to read a gel electrophoresis

Model the process of DNA sequencing through gel electrophoresis

Convert the DNA sequences into a protein by using an mRNA codon chart

 

 

Mader Chapter 16 PowerPoint

Mader Chapter 16 Notes

McGraw Hill Higher Learning Animations to Accompany Notes

Chapter 13 Genetic Engineering PowerPoint

 

15.1 Selective Breeding

Explain the purpose of selective breeding

Explain how people increase genetic variation

15.1 PowerPoint – 17 slides (selective breeding, hybridization, inbreeding, biotechnology)

Class Activity: The Perfect Cow

http://www.pbs.org/wnet/nature/lessons/the-perfect-cow/lesson-overview/1491/

Biologix Videos (30 minutes) Manipulating DNA

Homework: 15.1 Study Workbook

PBS Activity: Engineer a Crop (selective breeding & transgenic manipulation)

(Nature: Dogs That Changed the World – Selective Breeding Problems)

 

15.2 Recombinant DNA

Explain how scientists manipulate DNA

Describe the importance of recombinant DNA

Define transgenic and describe the usefulness of some transgenic organisms to humans

15.2 PowerPoint – 43 slides (polymerase chain reaction, recombinant DNA, plasmid, genetic marker, transgenic, clone)

**Biology Junction DNA Technology PPT slides 12-19 (Copying DNA & Cloning), slides 24-30 (Benefits of Genetic Engineering – Biotechnology)à Activities

• ·          MiniLab: Why Does Recombinant DNA Require Palindromes?
• ·          MiniLab: How is Splicing Wires Similar to Gene Splicing?
• Modeling Recombinant DNA Activity
• Biology Corner: DNA Analysis Simulating Recombination

Homework: 15.2 Study Workbook

Mader Notes: Chapter 16: 16.1 through A7

 

Whale Book Chapter 16 Notes: I Gene Manipulation – student version – teacher version

Whale Book Worksheets: Reinforcement & Study Guide page 61, page 62, page 63, page 64 & Chapter Assessment page 95 & page 96

DNA Restriction Animation: http://www.dnalc.org/resources/animations/restriction.html

Coloring Pages: 4-14 Recombinant DNA, 4-15 Genetic Engineering, 4-20 Transgenic Plants, 4-18 Gene Therapy

Activity: Modeling Recombinant DNA

Recombinant DNA Animations:

http://www.learner.org/courses/biology/archive/animations/hires/a_gmo1_h.html

http://www.dnalc.org/resources/animations/restriction.html

http://www.youtube.com/watch?v=ogjKNc5AMuY&feature=related

http://www.youtube.com/watch?v=x2jUMG2E-ic

 

PCR:

Mader Notes Chapter 16: 16.1 B

 

PCR Animation:

http://www.dnalc.org/resources/animations/pcr.html

http://www.youtube.com/watch?v=HMC7c2T8fVk&feature=fvwrel

Sumanas Inc Animation:

http://www.sumanasinc.com/webcontent/animations/content/pcr.html

http://www.sumanasinc.com/webcontent/animations/content/highthroughput.html

 

PCR Interactive:

http://learn.genetics.utah.edu/content/labs/pcr/

 

DNA Profiling:

http://www.insidedna.org.uk/content/?tag=profiling

Biology Corner Worksheet: Biotechnology Web Lesson – first page only (cloning) – some of this will mirror 2 genetics Utah worksheets

Genetics Utah: http://learn.genetics.utah.edu/content/tech/genetherapy/

• Cloning in Focus Web Quest

Using the Cloning in Focus Module, students complete a web quest to learn about cloning. (30 minutes)

• Let's Clone A Mouse, Mouse, Mouse ...

Somatic Cell Nuclear Transfer (SCNT) is a cloning method that involves transferring a nucleus from a somatic cell of the individual to be cloned to an enucleated egg. This activity simulates, step-by-step, the SCNT process used by researchers at the University of Hawaii.  (45 minutes)

 

15.3 Applications of Genetic Engineering

Describe the benefits of genetic engineering as they relate to agriculture and industry

Explain how recombinant DNA technology can improve human health

Summarize the process of DNA fingerprinting and explain its uses

15.3 PowerPoint – 42 slides (gene therapy, DNA microarray, DNA fingerprinting, forensics)

Continue Internet Activities:

à Biology Corner Worksheet: Biotechnology Web Lesson (second page – DNA Extraction & Gel Electrophoresis)

à NOVA: Cracking the Code of Life http://www.pbs.org/wgbh/nova/genome/ (no worksheet – just do it)

Activities: Sequence for Yourself, Explore a Stretch of Code

à DNA Detective: Human Identification – browse through the four tabs: profiling, family, murder, innocence

http://www.dnai.org/d/index.html?m=1

à NOVA: Create a DNA Fingerprint (biology corner short worksheet)

http://www.pbs.org/wgbh/nova/sheppard/analyze.html

 

Kim Brown Worksheets: DNA Fingerprinting Mysteries

 

Who Ate the Cheese? (biology corner worksheet)

 

Homework: 15.3 Study Workbook

 

learn moreà Sumanas Inc Animation:

http://www.sumanasinc.com/webcontent/animations/content/paternitytesting.html

http://www.sumanasinc.com/webcontent/animations/content/dnachips.html

 

Genetics Utah: http://learn.genetics.utah.edu/content/tech/genetherapy/

• Exploring Gene Therapy

Students navigate the Gene Therapy: Molecular Bandage? module to learn about gene therapy while completing a webquest. (45 minutes)

• Treatment, Enhancement, or Both?

Students consider potential gene therapy applications and categorize them as "enhancements" or "treatments" on a Venn diagram. (45 minutes)

 

gel electrophoresis lab

The objective of this experiment is to develop a basic understanding of electrophoretic theory, and gain “hands-on” familiarity with the procedures involved in agarose gel electrophoresis to separate biological molecules.

Sumanas Inc Animations

http://www.sumanasinc.com/webcontent/animations/content/gelelectrophoresis.html

 

15.4 Ethics and Impacts of Biotechnology

Describe some of the issues that relate to biotechnology

Identify some of the pros and cons of genetically modified food

Describe some of the ethical issues relating to biotechnology

15.4 PowerPoint – 25 slides

Genetics Utah: http://learn.genetics.utah.edu/content/tech/genetherapy/

• Positions, Beliefs and Values

Students review statements about gene therapy and determine how strongly they agree or disagree with each one. Students also write out the personal belief that leads them to their position. (20 minutes)

• The Bioethics of Gene Therapy

Students use a bioethical decision-making model to consider the ethical, legal and social implications of actual gene therapy trials. (60 minutes)

15.4 Study Workbook

 

Chapter 15 Test

 

Coloring Workbook Pages

• 4-14 Recombinant DNA
• 4-15 Genetic Engineering
• 4-16 Gene Probes
• 4-17 DNA Fingerprinting
• 1-18 Gene Therapy
• 4-19 Antisense Technology
• 4-20 Transgenic Plants